Another recent publication highlight!
The lab of Titia Sixma published a new study on how USP1/UAF1 can cleave ubiquitin chains on PCNA through an exo-cleavage mechanism.
Key findings:
- USP1/UAF1 prefers to cleave K63- and K48 on polyubiquitinated PCNA using exo-cleavage.
- USP1/UAF1 prefers to cleave ubiquitin-ubiquitin bond over PCNA-ubiquitin bond.
- UAF1 plays a minor role in exo-cleavage preference for K63-PCNA-UbN.
- – USP1/UAF1 uses a bind, cleave and release mechanism when acting on PCNA-UbN.
