Position 4

Regulation of DNA replication by PTMs during cell fate decisions.

PhD position in structural biology/biochemistry at the group of Prof Titia Sixma in the Division of Biochemistry at the Netherlands Cancer Institute, Amsterdam, the Netherlands.

What is the candidate going to do?

The candidate will study mechanisms of DUBs and E3 ubiquitin ligases in DNA replication in vitro using quantitative biochemistry and structural biology (cryo-EM and/or X-ray methods) to analyze allosteric regulation of these enzymes and their role in replication. Validation at the cellular level will be performed in collaboration with the Duxin and Altmeyer labs.

What does the candidate have to offer?
  • MSc in Structural Biology and/or Biochemistry‚Ķ

A highly motivated candidate with a strong background in cryo-EM, X-ray crystallography and/or protein would be preferred.

About the institution

The Netherlands Cancer Institute

The Netherlands Cancer Institute is a highly interactive international center of excellence with a high standard of biological research and outstanding research facilities. It is located in Amsterdam, with all its cultural amenities, close to Schiphol airport. Facilities include extensive protein expression and purification setups, a well-equipped macromolecular biophysics laboratory (part of InstructNL), and well-functioning cryo-EM (Jeol F2 200kV with a K2 detector) and high-throughput crystallisation robotics; these local facilities are coupled with regular access to Krios cryo-EM at NeCEN and to the ESRF, SLS and DLS synchrotrons.

Any questions?

Do you have any questions or do you require additional information? Please contact: Titia Sixma at t.sixma@nki.nl